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Therefore, we confirmed the anti-inflammatory efficacy of CBD isolated from Korean new hemp “Pink Pepper” in vitro, using RAW 264.7 cells for the inflammatory response induced by LPS and evaluated the protein and gene mechanism. In addition, whether CBD shows efficacy in acute inflammation was investigated in vivo using a carrageenan-induced paw edema mouse model. This study aimed to investigate the effect of CBD (cannabidiol) isolated from new hemp “Pink Pepper” on acute inflammation induced in mice and analyze the underlying mechanisms. The study aimed to utilize the anti-inflammatory effects of CBD on acute inflammation in mice as foundational research data for potential applications in human acute inflammation. As a result, the study intended to prove the necessity of the cultivation of “pink pepper” with a high CBD content and establish the value of the material. We investigated the anti-inflammatory effect and determined the industrial value of CBD isolated from “Pink Pepper”.
RAW 264.7 cells were maintained in Dulbecco’s Modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin, and streptomycin. Cells were grown at 37 °C in 5% CO2. The proliferation and viability of RAW 264.7 cells after sample treatment were confirmed through the Cell Counting Kit-8 (CCK-8) assay. The cells were seeded at 1 × 104 cells/well in 96-well cell culture plates and cultured for 18 h. Then, CBD sample solutions at various concentrations (1.25, 2.5, and 5 μM) were, respectively, added for 24 h. The CCK-8 solution (10 μL/well) was added to the cells in 96-well plates and measured according to the manufacturer’s protocol.
Inhibitory effect of CBD on mRNA expression of inflammatory factors. All samples except control were treated with 1 μg/mL of LPS. (A) iNOS; (B) COX-2; (C) IL-1β; (D) IL-6; and (E) TNF-α mRNA expression levels in groups treated with various concentrations of CBD compared with those in the LPS-only treatment group. Data represent the means ± SD in triplicate. p-value was calculated based on LPS-only data using ANOVA and Tukey’s post hoc test (** p < 0.01, *** p < 0.001).
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